APiX™ GlycoBiomarker Profiling Array Kit
| Glycans | ||||||||
|---|---|---|---|---|---|---|---|---|
| Lectin | Fucose | T antigen | Branched(1-6) LacNAc | Gal | LacNAc | Mannose | Sialic Acid | Chitobiose |
| AAL | 1 | |||||||
| BPL | 2 | |||||||
| LCA | 3 | |||||||
| PHA-L | 4 | 7 | ||||||
| RCA120 | 5 | 6 | ||||||
| SNA | 8 | |||||||
| WGA | * | 9 | ||||||
- Fuc (preferably (α1-6) linked
- Gal (ß1-3) GalNAc or terminal αGalNAc
- (α1-6) linked Fuc * WGA weakly binds Sialic Acid
- Gal(ß1-4) GlcNAc(ß1-6)-R
- Terminal ßGal as in Lactose/ LacNAc
- LacNAc (binding inhibited by terminal sialic acid)
- Terminal αMannose
- α2-6 linked Neu5 Ac
- (GlcNAc(ß1-4) GlcNAc)n repeats
Figure 2. Heatmap showing select glycan-glycoprotein ratios from disease vs. normal samples. Normal healthy, pancreatic cancer, and acute benign pancreatitis serum sample pools (n=4 each) were processed separately per the user protocol. Protein and lectin RFUs were obtained separately and used to generate glycan-glycoprotein ratios (log base 2 transformed) that normalize the relative abundance of glycans present to changes in underlying glycoprotein concentration. Data courtesy of Brian Haab, PhD of the Van Andel Research Institute and Songming Chen (present address: Imclone Pharmaceuticals).
Usage Notes
The APiX™ GlycoBiomarker Array Kit is designed to be used in conjunction with the SIMplex™ 64 multiplexing system (available separately from Gentel® Biosciences). The system is easy to use and is reusable. Eliminate broken slides, unreliable fasteners and leaky gaskets with the SIMplex™ multiplexing system.