Products Protein Array Kits Colony Surveillance Assays
Thursday, 21 July 2011 18:47

   Colony Surveillance Assay (CSA) Image

Continuous monitoring of SPF animals on a regular basis is considered the most effective method for colony health management. Gentel's Colony Surveillance Assays™ for Animal Health provide an easy to use and affordable system for performing routine colony serology in specific pathogen-free animals.

Detecting antibodies from previous exposure or illness to infectious agents is the most efficient and economical method for maintaining SPF status of research animal colonies. Depending on the type of infectious agent, animals may present symptoms of infection, mount an immune response, and clear the infection. During clearance, specific antibodies are generated to help contain the infection and prevent the animal from future infections. Some types of microbial pathogens are never completely cleared from the body, instead persisting in a latent state. Methods to directly detect virus can be very difficult and expensive, and often are unable to detect latent infections.

Applications and Features

Today’s proteomics research demands high performance reagents, scanning, and analysis. SilverQuant® detection surpass most alternative methods in assay sensitivity and provide equivalent reproducibility and dynamic range. The GR8 scanner features high-quality optics calibrated for access to both the high and low end colors that are inaccessible using conventional scanners but essential for proteomics research. AthenaQuant® Analysis Software combines spot-finding and spot-alignment tools with multiplexed protein quantitation, normalizationand analysis in a simple affordable, and easy-to-use package.


  CSA: Simian Basic CSA: Simian Expanded CSA: Simian Custom CSA: Simian Detection Kits
Number of viruses tested 7 11 Custom N/A
24 Samples 12-1007* 12-1008* 6-1054* 12-1006
96 samples 12-1007* 12-1008* 6-1054* 12-1010
Sample Assay Service 6-1027 6-1027 6-1027  
SRV1 (Simian Retrovirus Group 1) •
•  
SRV2 (Simian Retrovirus Group 2) • •
SRV4 (Simian Retrovirus Group 4) •
SRV5 (Simian Retrovirus Group 5) • • •  
STLV (Simian T Lymphotropic Virus) • • •  
SIV (Simian Immunodeficiency Virus) • • •  
B virus (Macacine herpesvirus 1) • • •  
HSV-1 (Herpes Simplex Type 1) • • •  
Measles (Paramyxovirus) • • •  
RRV (Rhesus rhadinovirus)   • •  
LCV (Lymphocryptovirus)   • •  
CMV (Cytomegalovirus)   • •  
SFV (Simian Foamy Virus)   •   •  
Additional requested antigens     •  

* requires use with CSA: Simian Detection Kits, Prod. 12-1006 or 12-1010

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How it works

CSA MethodClick to enlarge figure

Antigens are deposited into distict locations on the array. Sample serum is diluted and incubated on the array, in one of 24 subarrays per slide. If the animal has been exposed to a pathogen, specific IgG in the sera will bind to the antigen on the array. A biotinylated anti-species IgG will detect bound immune complexes, which is visualized with an anti-biotin gold antibody that is developed with the SilverQuant chromogenic detection reagents. Completed assays are scanned using the Gentel Proteomics Multi-System™ and analyzed with AthenaQuant™ software.

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